RNA酶抑制劑, 2000U

產品編號: BM-A3808

廠牌/產地: Biomatik, Canada

包裝:

• 2000U
• Concentration: 40U/μl
• Source: E. coli strain carrying the gene of RNase Inhibitor
• Unit Definition: One unit of this protein will inhibit 5 ng RNase A by 50%.
• Typical Reaction Conditions: RNase Inhibitor must be used at a final concentration of between 10-40u a 25μl reaction mix (This is dependent on the RNase contamination in the sample). For optimal RNase inhibition, a final concentration of 1mM DTT is required. 
• Storage: -20 °C

特點:

• Complete inhibition of RNase A, B and C;
• DNase/RNase and Nickase-Free;
• No inhibition of polymerase/transcriptase activity;
• Stable over a wide range pH, DTT concentrations and temperatures.

應用:

• RNA purification;
• cDNA preparation by reverse transcription;
• in vitro RNA transcription;
• in vitro protein synthesis