商品編號: | CUY21 SC |
商品名稱: | In Vivo 活體電穿孔儀 |
品牌廠牌: | NepaGene |
商品特點: | In Vivo, In Ovo, In Utero 應用: 活體電穿孔儀 |
The CUY21 SC is designed to output precise low voltage square wave pulses. The accurate and low voltage square wave pulses bring high transfection efficiency and less damage. Therefore, in the usage of this CUY21 electroporator, the low voltage range can cover almost all in vivo, in utero, in ovo and ex vivo applications unlike other electroporators.
The CUY21 SC can measure the electric current from the low ampere (1mA) up to 1.60A. Current is the major factor to obtain successful electroporation results. Initially the CUY21 SC was developed for the electroporation in which genes are delivered into the sensitive target such as mouse's brain, embryos, tissue slice and chick embryos. In those sensitive applications, low current is required to avoid the damage on a cell or brain etc. This CUY21 electroporator makes it possible to measure and display such current automatically. The output current information remarkably helps you optimize your electroporation experiments.
The CUY21 SC easily enables you to measure impedance (electric resistance) of the target sample and automatically displays it. The fluctuation in resistance value results in changes in current value and as a result it affects the reproducibility of experiments. In order to deliver the optimal current, you need to know the actual resistance of the target sample before electroporation. Because in vivo resistance is less stable than in vitro in general, the impedance value which is measured prior to in vivo electroporation is the important criteria to determine whether the experiment is going well.
For safety operation, the safety limit function is built in the CUY21 SC. To prevent unexpected electrical accidents, electroporation program stops immediately and automatically in case that the excess current (> 1.60A) flows.
應用:
• In Vivo Transfection
into mouse/rat brain, eye, muscle, skin, liver, kidney, testis, etc.
• In Utero Transfection
into mouse/rat embryos: cerebral cortex, hippocampus, spinal cord, etc.
• In Ovo Transfection
into chick embryos: neural tube, limb bud, digestive organ, etc.
• Ex-Vivo Transfection
into explants, brain slices, organs, whole embryos, etc.
規格:
DC Pulse Waveform | Square |
Impedance Measurement (Ohm Check) |
Max. 35Kohm |
Voltage | 0.1-99.9V (0.1V resolution) |
Pulse Length | 0.05-99.9ms (0.01ms resolution) |
Pulse Interval | 0.1-999.9ms (0.1ms resolution) |
Number of Pulses | 1-99 |
Safety Limit (High Current) | 1.60A |
Measured Output (Automatic) |
Voltage: 0.1-99.9V Current:1-999mA (1mA resolution), 1.00-1.60A (0.01A resolution) |
Memory | 99 Programs |
Dimensions, Weight | 360W x 380D x 180H mm, 12.3Kg |
Power | 115V or 220-240V, 50/60Hz |
活體用電極的選擇:
Transfection Targets |
Electrodes |
|
Chick and Quail Embryo |
Neural Tube (HH* stage 10) |
CUY610 series |
Chick Embryo (In Ovo) |
Optic Vesicle (HH stage 10) |
CUY610 series |
Chick Embryo |
Digestive Organ Epithelial Cell (HH stage 27-28) |
CUY520P5 |
Chick Embryo |
Gastrulation (HH stage 3-7) |
CUY701 series combination |
Mouse Embyo (In Utero) |
Cerebral Cortex, All Area (E12.5) |
CUY650P3 |
Mouse and Rat |
Neural Tube (Mouse: E9.5, Rat: E11.5) |
CUY520P15・P20・P25 |
Adult Mouse, Rat, Rabbit and Dog (In Vivo) |
CUY663 series |
|
Adult Mouse, Rat, Rabbit, Dog and Monkey |
Posterior Region of Leg |
CUY560-3, -5 (Injection) |
Adult Mouse and Rat |
Kidney |
CUY650P5 |
Adult Mouse and Rat |
Bladder |
CUY647P5X10 |
Adult Mouse and Rat |
Synovial Membrane |
CUY650P5, P10 |
Adult Mouse and Rat |
Brain |
CUY200S |
Newborn Mouse and Rat |
Retina (P0-P3) |
CUY675P3, P5 |
Newborn Mouse and Rat |
Brain Ventricle (P0-P3) |
CUY650P3, P5 |
Tissue Fragment |
CUY701 series combination |
|
Bee (In Vivo) |
CUY567 |
|
Silkworm |
CUY495P10 |
|
Xenopus Embryo |
Stage 12.5 |
CUY700P20 & CUY195P0.3 |
Fish |
CUY535, CUY536 |
|
Plant (In Vivo) |
訊詢單內容